A family spokesperson said in a statement, "We want to thank the amazing doctors at Cedars-Sinai Hospital for their expert care and kindness and we again want thank all of John's fans, friends and colleagues for all of the love and support they showed him during this difficult time".
Singleton had been on life support and the family made the decision to remove him from it today.
He is best known for his film, "Boyz N the Hood", for which he earned two Oscar nominations.
"In his private life, John was a loving and supporting father, son, brother, and friend who believed in higher education, black culture, old school music and the power of film," the family statement added.
Singleton is survived by his mother Sheila Ward, his father, Danny Singleton and his seven children Justice, Maasai, Hadar, Cleopatra, Selenesol, Isis, and Seven.
